In this webinar, we are going to study the activity of T7 DNA polymerase. By performing extension measurements at a constant tension, we will be able to detect and differentiate both polymerization and proof-reading events, which might be interspaced by pauses.
During this session, we will demonstrate that the LUMICKS C-Trap is an excellent tool to study the different modes of activity of a DNA polymerase while gaining insight into their occurrence, duration and probability with respect to the tension on the DNA template. Thereby, revealing many mechanistic aspects of the structure-function relationship of the molecular motor.
Instructor: Loïc Chaubet, Application Scientist
Background reading: Hoekstra, T. et al. Switching between Exonucleolysis and Replication by T7 DNA Polymerase Ensures High Fidelity. Biophysical Journal (https://www.cell.com/biophysj/fulltext/S0006-3495(17)30038-3) 2017, 112: 575-583