The Emory-Sage-SGC TREAT-AD center is taking an open science approach to promoting the study of under-studied AD genes and proteins by providing packages of information and reagents specific to targets to encourage their research as therapeutic AD targets by the research community. Target enabling packages, which includes antibody characterization reports, and an AD Informer Set, a set of high-quality compounds, both will enable biological evaluation and characterization of proteins with genetic linkage to AD.
YCharOS (antibody characterization through open science) is an open science initiative which aims at identifying high-quality antibodies for every human protein. By performing head-to-head comparisons of antibodies from various commercial sources by western blot, immunoprecipitation, and immunofluorescence, YCharOS prepares and publishes antibody characterization reports on an open-access repository. There are seven reports for targets nominated by the AMP-AD and TREAT-AD programs, with 50 target reports anticipated in total.
To assist the discovery of novel strategies that slow or halt AD pathology we have generated the AD Informer Set, the first iteration of which contains 171 chemical tools. The AD Informer Set includes compounds for targets that have AD-implicated biology, either well validated or recently suggested, and contains relevant information about the gene, compound data and activity, and associated AD phenotypes. The AD Informer Set will be shared with the scientific community at large to qualify assays, interrogate target validity in AD models, and to provide positive controls to aid the discovery of new chemical entities.
Learn more with Drs Riham Ayoubi and Alison Axtman, who will discuss strategies and methods for the selection of adequate cell lines for antibody characterization and the assembly and annotation of the AD Informer Set as an open science resource for studying AD biology.