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CryoEM Current Practices Webinar: Strategies and Highlights from the National CryoEM Centers
Determining structural mechanisms of allosteric regulation in ABCC family transporters using a remote cryo-EM pipeline

Tarjani Thaker, Ph.D. Scientist, Tomasiak Lab, University of Arizona

Hosted jointly by NCCAT, PNCC, & S2C2

Talk Abstract:
The democratization of cryo-EM has rapidly advanced thanks to the emergence of cryo-EM centers, making it possible for institutions lacking the infrastructure to pursue structural investigations by cryo-EM. Using the tools and expertise available at PNCC, our group at the University of Arizona has been able to successfully employ cryo-EM for structural studies on ABCC family transporters, in the process establishing an almost entirely remote pipeline for determining high-resolution structures. In this talk, I will share the strategies we have taken to build an efficient workflow that maximizes center resources to advance our understanding of the structure/function relationships in the allosteric regulation of ABC transporters. Our structures shed light on the role of post-translational modifications and lipid-like substrates in the functional regulation of dynamic and flexible membrane proteins involved in oxidative stress and multi-drug resistance which I will also present in this webinar, with an emphasis on data processing strategies used together with orthogonal tools to cross-validate our findings.

Dec 9, 2021 12:00 PM in Eastern Time (US and Canada)

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