Determining structural mechanisms of allosteric regulation in ABCC family transporters using a remote cryo-EM pipeline
Tarjani Thaker, Ph.D. Scientist, Tomasiak Lab, University of Arizona
Hosted jointly by NCCAT, PNCC, & S2C2
The democratization of cryo-EM has rapidly advanced thanks to the emergence of cryo-EM centers, making it possible for institutions lacking the infrastructure to pursue structural investigations by cryo-EM. Using the tools and expertise available at PNCC, our group at the University of Arizona has been able to successfully employ cryo-EM for structural studies on ABCC family transporters, in the process establishing an almost entirely remote pipeline for determining high-resolution structures. In this talk, I will share the strategies we have taken to build an efficient workflow that maximizes center resources to advance our understanding of the structure/function relationships in the allosteric regulation of ABC transporters. Our structures shed light on the role of post-translational modifications and lipid-like substrates in the functional regulation of dynamic and flexible membrane proteins involved in oxidative stress and multi-drug resistance which I will also present in this webinar, with an emphasis on data processing strategies used together with orthogonal tools to cross-validate our findings.